Peptide Dilution Calculator
Enter a stock solution's concentration and volume, plus the concentration you want to reach, to get the final total volume and how much diluent to add.
Results
- Final volume
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- Diluent to add
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How this calculator works
C1V1 = C2V2. This calculator solves for V2 (final volume) by multiplying the stock concentration by the stock volume, then dividing by the target concentration. Diluent to add is V2 minus the stock volume you started with.
How to dilute a peptide solution
- Find your stock solution's current concentration, in mg/mL or mcg/mL.
- Decide how much of that stock you're diluting, in mL.
- Decide the concentration you want to reach.
- The calculator gives you the final total volume and the diluent volume to add.
Worked example: 1 mL of a 10 mg/mL stock diluted to 2 mg/mL. Final volume = 10 × 1 ÷ 2 = 5 mL. Diluent to add = 5 − 1 = 4 mL of diluent to the 1 mL of stock.
Frequently asked questions
What formula does a peptide dilution calculator use?
C1V1 = C2V2 — the stock concentration times the stock volume equals the target concentration times the final volume. This calculator solves that equation for the final volume, then subtracts the stock volume you started with to get the diluent to add.
How do I dilute a peptide stock solution?
Enter your stock solution's concentration and the volume of it you're using, then enter the concentration you want to end up with. The calculator gives you the total final volume and how much diluent to add to the stock volume to reach it.
Why can't I enter a target concentration higher than my stock?
Dilution only ever lowers concentration — adding diluent increases volume, which can't raise the concentration of what's already in solution. To reach a higher concentration you'd need a more concentrated stock or a reconstitution with less water; see the peptide reconstitution calculator for that case.
Is this the same as the reconstitution calculator?
No. The reconstitution calculator turns a dry vial's mass and a water volume into a first concentration and a syringe draw for a dose. This dilution calculator starts from an already-liquid stock solution and calculates how to dilute it further to a lower target concentration — a common step in assay or serial-dilution setups.
Does the diluent have to be water?
This calculator only performs the arithmetic — mass and volume ratios — and doesn't specify a diluent. In practice, bacteriostatic water, sterile water, or a buffer are all common choices depending on the protocol; use whichever your protocol specifies.
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Educational tool only, not medical advice — verify with a licensed clinician. See the full medical disclaimer.